PT  - JOURNAL ARTICLE
AU  - Braulio Ayala García
AU  - Alma L. Fuentes-Farías
AU  - Gabriel Gutiérrez Ospina
TI  - Global DNA methylation, as estimated in blood samples, does not correlate with variations of body condition, anatomical features and hematological parameters in American bullfrogs (<em>Lithobates catesbeianus</em>) kept captive under distinct environmental conditions
AID  - 10.1101/156265
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 156265
4099  - http://biorxiv.org/content/early/2017/06/26/156265.short
4100  - http://biorxiv.org/content/early/2017/06/26/156265.full
AB  - Summary statement Previous reports support that Global DNA methylation, as estimated in blood samples, correlate with trait variability. Here we did not corroborate this assertion while testing in American bullfrogs that displayed divergence on some phenotypic traits.Abstract Different levels of Global DNA Methylation (GDM) could have facilitated the emergence of new species, without relying on gene mutations, through promoting ontogenetic phenotypic plasticity. If this assertion was correct, one could expect individuals of the same species living under distinct environmental conditions to be genetically similar, but having different GDM levels and being phenotypically divergent. We tested this presumption by studying the relationship between variability of functional morphological traits and GDM levels in American bullfrog (Lithobates catesbeianus), in green houses located in two geographical sites. Our analyses revealed that body linear morphometry, skull geometry, scaled mass index, packed cell volume and neutrophil counts differed significantly among males and females within and between localities. GDM, nonetheless, was rather similar among sex and locality groups. These results show that levels of GDM, at least under our experimental contexts, does not correlate with functional morphological trait variability.