RT Journal Article
SR Electronic
T1 Jellyfish oocyte Maturation Inducing Hormone and neuroendocrine regulation of reproduction
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 140160
DO 10.1101/140160
A1 Noriyo Takeda
A1 Yota Kon
A1 Gonzalo Quiroga Artigas
A1 Pascal Lapébie
A1 Carine Barreau
A1 Osamu Koizumi
A1 Takeo Kishimoto
A1 Kazunori Tachibana
A1 Evelyn Houliston
A1 Ryusaku Deguchi
YR 2017
UL http://biorxiv.org/content/early/2017/06/29/140160.abstract
AB Oocyte meiotic maturation is a critical process for sexually reproducing animals, and its core cytoplasmic regulators are highly conserved between species. In contrast, the few known Maturation Inducing Hormones (MIHs) that act on oocytes to initiate this process have highly variable molecular natures and their evolutionary relationships are poorly understood. Using the hydrozoan jellyfish species Clytia and Cladonema, which undergo oocyte maturation in response to dark-light/light-dark transitions, we identified from gonad transcriptome data amidated tetrapeptide sequences that could induce maturation of isolated oocytes at nanomolar concentrations. Antibody preabsorption experiments conclusively demonstrated that these W/RPRPamide-related neuropeptides account for endogenous MIH activity produced by isolated gonads. We further showed that the MIH neuropeptides are synthesised by neural-type cells in the gonad, are released following dark-light or light-dark transitions, and act on the oocyte surface. They are produced by male as well as female jellyfish and can trigger both sperm and egg release, suggesting a role in spawning coordination. We propose an evolutionary link between hydrozoan MIH and the neuropeptide hormones that regulate reproduction upstream of MIH release in bilaterian species.