PT - JOURNAL ARTICLE AU - André Veríssimo AU - Jean-Etienne Bassard AU - Alice Julien-Laferrière AU - Marie-France Sagot AU - Susana Vinga TI - MassBlast: A workflow to accelerate RNA-seq and DNA database analysis AID - 10.1101/131953 DP - 2017 Jan 01 TA - bioRxiv PG - 131953 4099 - http://biorxiv.org/content/early/2017/07/03/131953.short 4100 - http://biorxiv.org/content/early/2017/07/03/131953.full AB - Summary Current workflows for sequence analysis heavily depend on user input and manual curation. New specialized tools and methods are appearing all the time, but the actions required for a full analysis are disconnected and very time-consuming. The software we propose, MassBlast, combines BLAST+ and an automated workflow analysis to filter the results and significantly improve the annotation of multiple sequencing databases for exploring new biosynthetic pathways and new protein families, among other applications. MassBlast is fully configurable and reproducible.Availability and Implementation The MassBlast package is written in Ruby. Source code and releases are freely available from Github (https://github.com/averissimo/mass-blast) for all major platforms (Linux, MS Windows and OS X) under the GPLv3 license.Contact andre.verissimo{at}tecnico.ulisboa.pt