@article {Singh158998, author = {Irtisha Singh and Shih-Han Lee and Mehmet K Samur and Yu-Tzu Tai and Mehmet K Samur and Yu-Tzu Tai and Nikhil C Munshi and Christine Mayr and Christina S. Leslie}, title = {Widespread intronic polyadenylation diversifies immune cell transcriptomes}, elocation-id = {158998}, year = {2017}, doi = {10.1101/158998}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Alternative cleavage and polyadenylation (ApA) can generate mRNA isoforms with differences in 3{\textquoteright}UTR length without changing the coding region (CDR). However, ApA can also recognize intronic polyadenylation (IpA) signals to generate transcripts that lose part or all of the CDR. We analyzed 46 3{\textquoteright}-seq and RNA-seq profiles from normal human tissues, primary immune cells, and multiple myeloma (MM) samples and created an atlas of 4,927 high confidence IpA events. Up to 16\% of expressed genes in immune cells generate IpA isoforms, a majority of which are differentially used during B cell development or in different cellular environments, while MM cells display a striking loss of IpA isoforms expressed in plasma cells, their cell type of origin. IpA events can lead to truncated proteins lacking C-terminal functional domains. This can mimic ectodomain shedding through loss of transmembrane domains or alter the binding specificity of proteins with DNA-binding or protein-protein interaction domains, thus contributing to diversification of the transcriptome.}, URL = {https://www.biorxiv.org/content/early/2017/07/03/158998}, eprint = {https://www.biorxiv.org/content/early/2017/07/03/158998.full.pdf}, journal = {bioRxiv} }