TY - JOUR T1 - Oblique light-sheet tomography: fast and high resolution volumetric imaging of mouse brains JF - bioRxiv DO - 10.1101/132423 SP - 132423 AU - Arun Narasimhan AU - Kannan Umadevi Venkataraju AU - Judith Mizrachi AU - Dinu F. Albeanu AU - Pavel Osten Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/07/05/132423.abstract N2 - Present light sheet fluorescence microscopes lack the wherewithal to image the whole brain (large tissues) with uniform illumination/detection parameters and high enough resolution to provide an understanding of the various aspects of neuroanatomy. To overcome this, we developed an oblique version of the light sheet microscope (Oblique Light Sheet Tomography, OLST) that includes a high magnification objective and serial sectioning, for volumetric imaging of the whole mouse brain at high spatial resolution at constant illumination/detection. We developed a novel gelatin based re-embedding procedure that makes the cleared brain rigid so that it can sectioned using our integrated microtome. Here, we characterize OLST and show that it can be used to observe dendritic morphology, spines and follow axons over a few mm in the mouse brain. ER -