RT Journal Article SR Electronic T1 Enhanced skeletal muscle ribosome biogenesis, yet attenuated mTORC1 and ribosome biogenesis-related signalling, with concurrent versus single-mode resistance training JF bioRxiv FD Cold Spring Harbor Laboratory SP 115212 DO 10.1101/115212 A1 Jackson J. Fyfe A1 David J. Bishop A1 Jonathan D. Bartlett A1 Erik D. Hanson A1 Mitchell J. Anderson A1 Andrew P. Garnham A1 Nigel K. Stepto YR 2017 UL http://biorxiv.org/content/early/2017/07/06/115212.abstract AB Combining endurance training with resistance training (RT) may attenuate skeletal muscle hypertrophic adaptation versus RT alone; however, the underlying mechanisms are unclear. We investigated changes in markers of ribosome biogenesis, a process linked with skeletal muscle hypertrophy, following concurrent training versus RT performed alone. Twenty-three recreationally-active males underwent eight weeks of RT, either performed alone (RT group, n = 8), or combined with either high-intensity interval training (HIT+RT group, n = 8), or moderate-intensity continuous training (MICT+RT group, n = 7). Muscle samples (vastus lateralis) were obtained before training, and immediately before, 1 h and 3 h after the final training session. Training-induced changes in basal expression of the 45S ribosomal RNA (rRNA) precursor, and 5.8S and 28S mature rRNAs, were greater with concurrent training versus RT alone. However, during the final training session, RT induced further increases in both mTORC1 (p70S6K1 and rps6 phosphorylation) and 45S rRNA transcription-related signalling (TIF-1A and UBF phosphorylation) versus concurrent training. These data suggest that when performed in a training-accustomed state, RT preferentially induces mTORC1 and ribosome biogenesis-related signalling in human skeletal muscle versus concurrent training; however, changes in skeletal muscle ribosome biogenesis markers were more favourable following concurrent training versus RT performed alone.