RT Journal Article
SR Electronic
T1 LM6-M: a high avidity rat monoclonal antibody to pectic α-1,5-L-arabinan
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 161604
DO 10.1101/161604
A1 Valérie Cornuault
A1 Fanny Buffetto
A1 Susan E Marcus
A1 Marie-Jeanne Crépeau
A1 Fabienne Guillon
A1 Marie-Christine Ralet
A1 J Paul Knox
YR 2017
UL http://biorxiv.org/content/early/2017/07/10/161604.abstract
AB 1,5-arabinan is an abundant structural feature of side chains of pectic rhamnogalacturonan-I which is a matrix constituent of plant cell walls. The study of arabinan in cells and tissues is driven by putative roles for this polysaccharide in the generation of cell wall and organ mechanical properties. The biological function(s) of arabinan is still uncertain and high quality molecular tools are required to detect its occurrence and monitor its dynamics. Here we report a new rat monoclonal antibody, LM6-M, similar in specificity to the published rat monoclonal antibody LM6 (Willats et al. (1998) Carbohydrate Research 308: 149-152). LM6-M is of the IgM immunoglobulin class and has a higher avidity for α-1-5-L-arabinan than LM6. LM6-M displays high sensitivity in its detection of arabinan in in-vitro assays such as ELISA and epitope detection chromatography and in in-situ analyses.AraArabinoseBSABovine Serum AlbuminGalGalactoseGalAGalacturonic acidEDCEpitope detection chromatographyELISAEnzyme-Linked Immunosorbent AssaymAbMonoclonal antibodyPBSPhosphate-buffered salineRhaRhamnoseRG-IRhamnogalacturonan-I