RT Journal Article
SR Electronic
T1 Evolutionary rewiring of the human regulatory network by waves of genome expansion
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 164434
DO 10.1101/164434
A1 Davide Marnetto
A1 Federica Mantica
A1 Ivan Molineris
A1 Elena Grassi
A1 Igor Pesando
A1 Paolo Provero
YR 2017
UL http://biorxiv.org/content/early/2017/07/17/164434.abstract
AB Genome expansion is believed to be an important driver of the evolution of gene regulation. To investigate the role of newly arising sequence in rewiring the regulatory network we estimated the age of each region of the human genome by applying maximum parsimony to genome-wide alignments with 100 vertebrates. We then studied the age distribution of several types of functional regions, with a focus on regulatory elements. The age distribution of regulatory elements reveals the extensive use of newly formed genomic sequence in the evolution of regulatory interactions. Many transcription factors have expanded their repertoire of targets through waves of genomic expansions that can be traced to specific evolutionary times. Repeated elements contributed a major part of such expansion: many classes of such elements are enriched in binding sites of one or a few specific transcription factors, whose binding sites are localized in specific portions of the element and characterized by distinctive motif words. These features suggest that the binding sites were available as soon as the new sequence entered the genome, rather than being created later by accumulation of point mutations. By comparing the age of regulatory regions to the evolutionary shift in expression of nearby genes we show that rewiring through genome expansion played an important role in shaping the human regulatory network.