TY - JOUR T1 - <em>Drosophila</em> Kruppel homolog 1 represses lipolysis through interaction with dFOXO JF - bioRxiv DO - 10.1101/165456 SP - 165456 AU - Ping Kang AU - Kai Chang AU - Ying Liu AU - Mark Bouska AU - Galina Karashchuk AU - Rachel Thakore AU - Wenjing Zheng AU - Stephanie Post AU - Colin S. Brent AU - Sheng Li AU - Marc Tatar AU - Hua Bai Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/07/18/165456.abstract N2 - Transcriptional coordination is a vital process contributing to metabolic homeostasis. As one of the key nodes in the metabolic network, the forkhead transcription factor FOXO has been shown to interact with diverse transcription co-factors and integrate signals from multiple pathways to control metabolism, oxidative stress response, and cell cycle. Recently, insulin/FOXO signaling has been implicated in the regulation of insect development via the interaction with insect hormones, such as ecdysone and juvenile hormone. In this study, we identified an interaction between dFOXO and the zinc finger transcription factor Kruppel homolog 1 (Kr-h1), one of the key players in juvenile hormone signaling in Drosophila. We found that Kr-h1 mutants have reduced triglyceride storage, decreased insulin signaling and delayed larval development. Notably, Kr-h1 physically and genetically interacts with dFOXO in vitro and in vivo to regulate the transcriptional activation of adipose lipase brummer (bmm). The transcriptional co-regulation by Kr-h1 and dFOXO may represent a broad mechanism by which Kruppel-like factors integrate with insulin signaling to maintain metabolic homeostasis and coordinate organism growth. ER -