TY - JOUR T1 - Detailed analyses of stall force generation in <em>Mycoplasma mobile</em> gliding JF - bioRxiv DO - 10.1101/142133 SP - 142133 AU - Masaki Mizutani AU - Isil Tulum AU - Yoshiaki Kinosita AU - Takayuki Nishizaka AU - Makoto Miyata Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/07/20/142133.abstract N2 - Mycoplasma mobile is a bacterium that uses a unique mechanism to glide on solid surfaces at a velocity of up to 4.5 µm/s. Its gliding machinery comprises hundreds of units that generate the force for gliding based on the energy derived from ATP; the units catch and pull on sialylated oligosaccharides fixed to solid surfaces. In the present study, we measured the stall force of wild-type and mutant strains of M. mobile carrying a bead manipulated using optical tweezers. The strains that had been enhanced for binding exhibited weaker stall forces than the wild-type strain, indicating that stall force is related to force generation rather than to binding. The stall force of the wild-type strain decreased linearly from 113 to 19 pN following the addition of 0–0.5 mM free sialyllactose (a sialylated oligosaccharide), with a decrease in the number of working units. Following the addition of 0.5 mM sialyllactose, the cells carrying a bead loaded using optical tweezers exhibited stepwise movements with force increments. The force increments ranged from 1 to 2 pN. Considering the 70-nm step size, this small unit force may be explained by the large gear ratio involved in the M. mobile gliding machinery.SIGNIFICANCE Mycoplasma is a genus of bacteria that parasitizes animals. Dozens of Mycoplasma species glide over the tissues of their hosts during infection. The gliding machinery of Mycoplasma mobile, the fastest species, includes intracellular motors and hundreds of legs on the cell surface. In the present study, we precisely measured force generation using a highly focused laser beam arrangement (referred to as optical tweezers) under various conditions. The measurements obtained in this study suggest that the rapid gliding exhibited by M. mobile arises from the large gear ratio of its gliding machinery. ER -