@article {Tapia Pacheco168740, author = {M{\'o}nica Tapia Pacheco and Pierre Baudot and Martial A. Dufour and Christine Formisano-Tr{\'e}ziny and Simone Temporal and Manon Lasserre and Jean Gabert and Kazuto Kobayashi and Jean-Marc Goaillard}, title = {Information topology of gene expression profile in dopaminergic neurons}, elocation-id = {168740}, year = {2017}, doi = {10.1101/168740}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Extracting high-degree interactions and dependences between variables (pairs, triplets, {\textellipsis} k-tuples) is a challenge posed by all omics approaches1, 2. Here we used multivariate mutual information (Ik) analysis3 on single-cell retro-transcription quantitative PCR (sc-RTqPCR) data obtained from midbrain neurons to estimate the k-dimensional topology of their gene expression profiles. 41 mRNAs were quantified and statistical dependences in gene expression levels could be fully described for 21 genes: Ik analysis revealed a complex combinatorial structure including modules of pairs, triplets (up to 6-tuples) sharing strong positive, negative or zero Ik, corresponding to co-varying, clustering and independent sets of genes, respectively. Therefore, Ik analysis simultaneously identified heterogeneity (negative Ik) of the cell population under study and regulatory principles conserved across the population (homogeneity, positive Ik). Moreover, maximum information paths enabled to determine the size and stability of such transcriptional modules. Ik analysis represents a new topological and statistical method of data analysis.}, URL = {https://www.biorxiv.org/content/early/2017/07/26/168740}, eprint = {https://www.biorxiv.org/content/early/2017/07/26/168740.full.pdf}, journal = {bioRxiv} }