RT Journal Article
SR Electronic
T1 GUIDES: sgRNA design for loss-of-function screens
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 168849
DO 10.1101/168849
A1 Joshua A. Meier
A1 Feng Zhang
A1 Neville E. Sanjana
YR 2017
UL http://biorxiv.org/content/early/2017/07/26/168849.abstract
AB GUIDES (Graphical User Interface for DNA Editing Screens) is a web-based tool for the design of custom, large-scale CRISPR libraries for loss-of-function screens in human and mouse. GUIDES combines multi-tissue RNA-sequencing data to target expressed exons, protein annotation to target functional domains, sophisticated on-target and off-target guide RNA scoring and other optimizations to create CRISPR libraries directly from a list of genes without requiring any programming expertise.