TY - JOUR T1 - INO80 Chromatin Remodelling Coordinates Metabolic Homeostasis with Cell Division JF - bioRxiv DO - 10.1101/169128 SP - 169128 AU - Graeme J. Gowans AU - Alicia N. Schep AU - Ka Man Wong AU - Devin A. King AU - William J. Greenleaf AU - Ashby J. Morrison Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/07/27/169128.abstract N2 - Adaptive survival requires the coordination of nutrient availability with expenditure of cellular resources. For example, in nutrient-limited environments, 50% of all S. cerevisiae genes synchronize and exhibit periodic bursts of expression in coordination with respiration and cell division in the Yeast Metabolic Cycle (YMC). Despite the importance of metabolic and proliferative synchrony, the majority of YMC regulators are currently unknown. Here we demonstrate that the INO80 chromatin-remodelling complex is required to coordinate respiration and cell division with periodic gene expression. Specifically, INO80 mutants have severe defects in oxygen consumption and promiscuous cell division that is no longer coupled with metabolic status. In mutant cells, chromatin accessibility of periodic genes, including TORC-responsive genes, is relatively static, concomitant with severely attenuated gene expression. Collectively, these results reveal that the INO80 complex mediates metabolic signaling to chromatin in order to restrict proliferation to metabolically optimal states. ER -