RT Journal Article
SR Electronic
T1 The nucleoid as a scaffold for the assembly of bacterial signaling complexes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 169912
DO 10.1101/169912
A1 Audrey Moine
A1 Leon Espinosa
A1 Eugenie Martineau
A1 Deborah Byrne
A1 Emanuele G. Biondi
A1 Eugenio Notomista
A1 Matteo Brilli
A1 Virginie Molle
A1 Tâm Mignot
A1 Emilia M.F. Mauriello
YR 2017
UL http://biorxiv.org/content/early/2017/07/28/169912.abstract
AB The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. In this work, we show that FrzCD directly binds to the nucleoid with its N-terminal positively charged tail and recruits active signaling complexes at this location. The FrzCD binding to the DNA leads to the formation of multiple distributed clusters that explore constrained areas. This supra-molecular organization is required for cooperative interactions between clustered receptors, in turn important for the modulation of bacterial social behaviors.