PT  - JOURNAL ARTICLE
AU  - Muller Heloise
AU  - Scolari F. Vittore
AU  - Mercy Guillaume
AU  - Agier Nicolas
AU  - Descorps-Declere Stephane
AU  - Fischer Gilles
AU  - Mozziconacci Julien
AU  - Koszul Romain
TI  - Redesigning chromosomes to optimize conformation capture (Hi-C) assays
AID  - 10.1101/169847
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 169847
4099  - http://biorxiv.org/content/early/2017/07/28/169847.short
4100  - http://biorxiv.org/content/early/2017/07/28/169847.full
AB  - In all chromosome conformation capture based experiments the accuracy with which contacts are detected varies considerably because of the uneven distribution of restriction sites along genomes. Here, we redesigned and reassembled in yeast a 145kb region with regularly spaced restriction sites for various enzymes. Thanks to this design, we enhanced the signal to noise ratio and improved the visibility of the entire region as well as our understanding of Hi-C data, while opening new perspectives to future studies.