RT Journal Article
SR Electronic
T1 Fitness effects of <em>cis</em>-regulatory variants in the <em>Saccharomyces cerevisiae TDH3</em> promoter
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 154039
DO 10.1101/154039
A1 Fabien Duveau
A1 William Toubiana
A1 Patricia J. Wittkopp
YR 2017
UL http://biorxiv.org/content/early/2017/08/02/154039.abstract
AB Variation in gene expression is widespread within and between species, but fitness consequences of this variation are generally unknown. Here we use mutations in the Saccharomyces cerevisiae TDH3 promoter to assess how changes in TDH3 expression affect cell growth. From these data, we predict the fitness consequences of de novo mutations and natural polymorphisms in the TDH3 promoter. Nearly all mutations and polymorphisms in the TDH3 promoter were found to have no significant effect on fitness in the environment assayed, suggesting that the wild type allele of this promoter is robust to the effects of most new cis-regulatory mutations.