RT Journal Article
SR Electronic
T1 C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 171819
DO 10.1101/171819
A1 Xin D. Gao
A1 Li-Chun Tu
A1 Aamir Mir
A1 Job Dekker
A1 Scott A. Shaffer
A1 Lihua Julie Zhu
A1 Scot A. Wolfe
A1 Erik J. Sontheimer
YR 2017
UL http://biorxiv.org/content/early/2017/08/02/171819.abstract
AB Mapping proteomic composition at distinct genomic loci and subnuclear landmarks in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, facilitating annotation of these factors and their roles in nuclear and chromosome biology.