TY - JOUR T1 - C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2 JF - bioRxiv DO - 10.1101/171819 SP - 171819 AU - Xin D. Gao AU - Li-Chun Tu AU - Aamir Mir AU - Job Dekker AU - Scott A. Shaffer AU - Lihua Julie Zhu AU - Scot A. Wolfe AU - Erik J. Sontheimer Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/08/02/171819.abstract N2 - Mapping proteomic composition at distinct genomic loci and subnuclear landmarks in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, facilitating annotation of these factors and their roles in nuclear and chromosome biology. ER -