RT Journal Article
SR Electronic
T1 Pan-embryo cell dynamics of germlayer formation in zebrafish
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 173583
DO 10.1101/173583
A1 Gopi Shah
A1 Konstantin Thierbach
A1 Benjamin Schmid
A1 Anna Reade
A1 Ingo Roeder
A1 Nico Scherf
A1 Jan Huisken
YR 2017
UL http://biorxiv.org/content/early/2017/08/08/173583.abstract
AB Cell movements are coordinated across spatio-temporal scales to achieve precise positioning of organs during vertebrate gastrulation. In zebrafish, mechanisms governing such morphogenetic movements have so far only been studied within a local region or a single germlayer. Here, we present pan-embryo analyses of fate specification and dynamics of all three germlayers simultaneously within a gastrulating embryo, showing that cell movement characteristics are predominantly determined by its position within the embryo, independent of its germlayer identity. The spatially confined fate specification establishes a distinct distribution of cells in each germlayer during early gastrulation. The differences in the initial distribution are subsequently amplified by a unique global movement, which organizes the organ precursors along the embryonic body axis, giving rise to the blueprint of organ formation.