RT Journal Article
SR Electronic
T1 nextPARS: Parallel probing of RNA structures in Illumina
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 174144
DO 10.1101/174144
A1 Ester Saus
A1 Jesse Willis
A1 Leszek P. Pryszcz
A1 Heinz Himmelbauer
A1 Toni Gabaldón
YR 2017
UL http://biorxiv.org/content/early/2017/08/09/174144.abstract
AB RNA molecules play important roles in virtually every cellular process. These functions are often mediated through the adoption of specific structures that enable RNAs to interact with other molecules. Thus, determining the secondary structures of RNAs is central to understanding their function and evolution. In recent years several sequencing-based approaches have been developed that allow probing structural features of thousands of RNA molecules present in a sample. Here, we describe nextPARS, a novel Illumina-based implementation of in-vitro parallel probing of RNA structures. Our approach achieves comparable accuracy to previous implementations, while enabling higher throughput and sample multiplexing.