RT Journal Article
SR Electronic
T1 Plasticity of the <em>MFS1</em> promoter leads to multi drug resistance in the wheat pathogen <em>Zymoseptoria tritici</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 174722
DO 10.1101/174722
A1 Selim Omrane
A1 Colette Audéon
A1 Amandine Ignace
A1 Clémentine Duplaix
A1 Lamia Aouini
A1 Gert Kema
A1 Anne-Sophie Walker
A1 Sabine Fillinger
YR 2017
UL http://biorxiv.org/content/early/2017/08/10/174722.abstract
AB The ascomycete Zymoseptoria tritici is the causal agent of septoria leaf blotch on wheat. Disease control relies mainly on resistant wheat cultivars and on fungicide applications. The fungus displays a high potential to circumvent both methods. Resistance against all unisite fungicides has been observed over decades. A different type of resistance has emerged among wild populations with multi-drug resistant (MDR) strains. Active fungicide efflux through overexpression of the major-facilitator gene, MFS1, explains this emerging resistance mechanism. In this study, we identified as responsible mutations three types of inserts in the MFS1 promoter, two of which harboring potential transcription factor binding sites. We show, that type I insertion leads to MFS1 overexpression and consequently to MDR. Interestingly, all three inserts correspond to repeated elements of the Z. tritici genome.These results underline the plasticity of repeated elements leading to fungicide resistance in Z. tritici and which contribute to its adaptive potential.