RT Journal Article
SR Electronic
T1 Fast, robust and precise 3D localization for arbitrary point spread functions
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 172643
DO 10.1101/172643
A1 Yiming Li
A1 Markus Mund
A1 Philipp Hoess
A1 Ulf Matti
A1 Bianca Nijmeijer
A1 Vilma Jimenez Sabinina
A1 Jan Ellenberg
A1 Ingmar Schoen
A1 Jonas Ries
YR 2017
UL http://biorxiv.org/content/early/2017/08/10/172643.abstract
AB We present a fitter for 3D single-molecule localization of arbitrary, experimental point spread functions (PSFs) that reaches minimum uncertainty for EMCCD and sCMOS cameras, and achieves more than 105 fits/s. We provide tools to robustly model experimental PSFs and correct for depth induced aberrations, which allowed us to achieve an unprecedented 3D resolution with engineered astigmatic PSFs, and acquire high quality 3D superresolution images even on standard microscopes without 3D optics.