RT Journal Article SR Electronic T1 X10 Expansion Microscopy Enables 25 nm Resolution on Conventional Microscopes JF bioRxiv FD Cold Spring Harbor Laboratory SP 172130 DO 10.1101/172130 A1 Sven Truckenbrodt A1 Manuel Maidorn A1 Dagmar Crzan A1 Hanna Wildhagen A1 Selda Kabatas A1 Silvio O. Rizzoli YR 2017 UL http://biorxiv.org/content/early/2017/08/11/172130.abstract AB We present here an improved protocol for expansion microscopy, which increases the expansion factor from ~4x to ~10x. This protocol, which we termed X10 microscopy, achieves multi-colour imaging at a resolution of at least 30 nm, using off-the-shelf reagents and conventional epifluorescence microscopes. This enables a level of imaging detail rivalling that of much more challenging methods, such as STED, STORM and iExM, in both cell cultures and tissues.