RT Journal Article SR Electronic T1 3D single-molecule super-resolution microscopy with a tilted light sheet JF bioRxiv FD Cold Spring Harbor Laboratory SP 135699 DO 10.1101/135699 A1 Anna-Karin Gustavsson A1 Petar N. Petrov A1 Maurice Y. Lee A1 Yoav Shechtman A1 W. E. Moerner YR 2017 UL http://biorxiv.org/content/early/2017/08/14/135699.abstract AB Tilted light sheet microscopy with 3D point spread functions (TILT3D) combines a novel, tilted light sheet illumination strategy with long axial range point spread functions (PSFs) for low-background, 3D super-localization of single molecules as well as 3D super-resolution imaging in thick cells. Because the axial positions of the single emitters are encoded in the shape of each single-molecule image rather than in the position or thickness of the light sheet, the light sheet need not be extremely thin. TILT3D is built upon a standard inverted microscope and has minimal custom parts. The result is simple and flexible 3D super-resolution imaging with tens of nm localization precision throughout thick mammalian cells. We validated TILT3D for 3D super-resolution imaging in mammalian cells by imaging mitochondria and the full nuclear lamina using the double-helix PSF for single-molecule detection and the recently developed Tetrapod PSFs for fiducial bead tracking and live axial drift correction.