PT  - JOURNAL ARTICLE
AU  - Andrea Zelmer
AU  - Rachel Tanner
AU  - Elena Stylianou
AU  - Sheldon Morris
AU  - Angelo Izzo
AU  - Ann Williams
AU  - Sally Sharpe
AU  - Ilaria Pepponi
AU  - Barry Walker
AU  - David A. Hokey
AU  - Helen McShane
AU  - Michael Brennan
AU  - Helen Fletcher
TI  - <em>Ex vivo</em> mycobacterial growth inhibition assay (MGIA) for tuberculosis vaccine testing - a protocol for mouse splenocytes
AID  - 10.1101/020560
DP  - 2015 Jan 01
TA  - bioRxiv
PG  - 020560
4099  - http://biorxiv.org/content/early/2015/06/07/020560.short
4100  - http://biorxiv.org/content/early/2015/06/07/020560.full
AB  - The testing of vaccines for tuberculosis is costly and time-consuming, and dependent on preclinical animal challenge models and clinical trials. We have recently developed a mycobacterial growth inhibition assay (MGIA) to test vaccine efficacy ex vivo. This assay measures the summative effect of the host immune response and may serve as a novel tool to facilitate vaccine testing. It has generated much interest recently, and to facilitate technology transfer and reproducibility between laboratories, we here describe a detailed protocol for an ex vivo MGIA in mouse splenocytes.