RT Journal Article
SR Electronic
T1 A Molecular Classification of Human Mesenchymal Stromal Cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 024414
DO 10.1101/024414
A1 Florian Rohart
A1 Elizabeth Mason
A1 Nicholas Matigian
A1 Rowland Mosbergen
A1 Othmar Korn
A1 Tyrone Chen
A1 Suzanne Butcher
A1 Jatin Patel
A1 Kerry Atkinson
A1 Kiarash Khosrotehrani
A1 Nicholas M Fisk
A1 Kim-Anh Lê Cao
A1 Christine A Wells
YR 2015
UL http://biorxiv.org/content/early/2015/08/11/024414.abstract
AB Mesenchymal stromal cells (MSC) are widely used, isolated from a variety of tissues and increasingly adopted for cell therapy, but the identity of these cells is poorly defined and commonalities between MSC from different tissues sources is controversial. Here we undertook a comprehensive review of all public MSC expression studies to assess whether cells derived from different sources shared any common molecular attributes. In doing so, we discovered an over-arching transcriptional phenotype shared by a wide variety of MSC, freshly isolated or cultured cells, and under a variety of growth conditions. We developed a modified variable selection protocol that included cross platform normalisation, and assessment of the selected gene stability and informativeness. A 16-gene signature classified MSC with >97% accuracy, discriminating these from fibroblasts, other adult stem/progenitor cell types and differentiated cells. The genes form part of a protein-interaction network, and mutations in more than 65% of this network were associated with Mendelian disorders of skeletal growth or metabolism. The signature and accompanying datasets are provided as a community resource at www.stemformatics.org resource, and the method is available from the CRAN repository.