RT Journal Article
SR Electronic
T1 Extensive translation of small ORFs revealed by polysomal ribo-Seq
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 002998
DO 10.1101/002998
A1 Julie L. Aspden
A1 Ying Chen Eyre-Walker
A1 Rose J. Philips
A1 Michele Brocard
A1 Unum Amin
A1 Juan Couso
YR 2014
UL http://biorxiv.org/content/early/2014/02/24/002998.abstract
AB Thousands of small Open Reading Frames (smORFs) encoding small peptides of fewer than 100 amino acids exist in our genomes. Examples of functional smORFs have been characterised in a few species but the actual number of translated smORFs, and their molecular, functional and evolutionary features are not known. Here we present a genome-wide assessment of smORF translation by ribosomal profiling of polysomal fractions. This ‘polysomal ribo-Seq’ suggests that smORFs are translated at the same level and in the same relative numbers (80%) as normal proteins. The smORF peptides appear widely conserved, show activity in cells, and display a putative amino acid signature. These findings reinforce the idea that smORFs are an abundant and fundamental genome component, displaying features usually attributed to canonical proteins, including high translation levels, biological function, amino acid sequence specificity and cross-species conservation.