TY - JOUR T1 - Scan-o-matic: high-resolution microbial phenomics at a massive scale JF - bioRxiv DO - 10.1101/031443 SP - 031443 AU - Martin Zackrisson AU - Johan Hallin AU - Lars-Göran Ottosson AU - Peter Dahl AU - Esteban Fernandez-Parada AU - Erik Ländström AU - Luciano Fernandez-Ricaud AU - Petra Kaferle AU - Andreas Skyman AU - Stig Omholt AU - Uros Petrovic AU - Jonas Warringer AU - Anders Blomberg Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/11/12/031443.abstract N2 - The capacity to map traits over large cohorts of individuals – phenomics – lags far behind the explosive development in genomics. For microbes the estimation of growth is the key phenotype. We introduce an automated microbial phenomics framework that delivers accurate and highly resolved growth phenotypes at an unprecedented scale. Advancements were achieved through introduction of transmissive scanning hardware and software technology, frequent acquisition of precise colony population size measurements, extraction of population growth rates from growth curves and removal of spatial bias by reference-surface normalization. Our prototype arrangement automatically records and analyses 100,000 experiments in parallel. We demonstrate the power of the approach by extending and nuancing the known salt defence biology in baker’s yeast. The introduced framework will have a transformative impact by providing high-quality microbial phenomics data for extensive cohorts of individuals and generating well-populated and standardized phenomics databases. ER -