PT  - JOURNAL ARTICLE
AU  - Frederick B. Shipley
AU  - Christopher M. Clark
AU  - Mark J. Alkema
AU  - Andrew M. Leifer
TI  - Simultaneous optogenetic manipulation and calcium imaging in freely moving <em>C. elegans</em>
AID  - 10.1101/000943
DP  - 2014 Jan 01
TA  - bioRxiv
PG  - 000943
4099  - http://biorxiv.org/content/early/2014/04/02/000943.short
4100  - http://biorxiv.org/content/early/2014/04/02/000943.full
AB  - A fundamental goal of systems neuroscience is to probe the dynamics of neural activity that drive behavior. Here we present an instrument to simultaneously manipulate neural activity via Channelrhodopsin, monitor neural response via GCaMP3, and observes behavior in freely moving C. elegans. We use the instrument to directly observe the relation between sensory stimuli, interneuron activity and locomotion in the mechanosensory circuit. Now published as: Front Neural Circuits 8:28, doi:10.3389/fncir.2014.00028