The rise of antibiotic-resistant bacteria has led to an urgent need for rapid detection of drug
resistance in clinical samples, and improvements in global surveillance. Here we show how …

Routine full characterization of Mycobacterium tuberculosis is culture based, taking many
weeks. Whole-genome sequencing (WGS) can generate antibiotic susceptibility profiles to …

Background Slow and cumbersome laboratory diagnostics for Mycobacterium tuberculosis
complex (MTBC) risk delayed treatment and poor patient outcomes. Whole-genome …

Whole-genome sequencing offers new insights into the evolution of bacterial pathogens
and the etiology of bacterial disease. Staphylococcus aureus is a major cause of bacteria-…

Bacterial virulence is a multifaceted trait where the interactions between pathogen and host
factors affect the severity and outcome of the infection. Toxin secretion is central to the …

Background Staphylococcal protein A (spa) is an important virulence factor which enables
Staphylococcus aureus to evade host immune responses. Genotypes known as “spa-types”, …

We developed a low-cost and reliable method of DNA extraction from as little as 1 ml of
early positive mycobacterial growth indicator tube (MGIT) cultures that is suitable for whole-…

Staphylococcus aureus is a commensal that can also cause invasive infection. Reports suggest
that nasal cocolonization occurs rarely, but the resources required to sequence multiple …

Background Carriage of Staphylococcus aureus is a risk for infections. Targeted decolonization
reduces postoperative infections but depends on accurate screening. Aim To compare …

DNA sequence analysis and genetic mapping of loci from mating-type-specific chromosomes
of the smut fungus Microbotryum violaceum demonstrated that the nonrecombining mating-…