Crucial transitions in cancer—including tumor initiation, local expansion, metastasis, and
therapeutic resistance—involve complex interactions between cells within the dynamic tumor …

Human genomes are diploid and, for their complete description and interpretation, it is
necessary not only to discover the variation they contain but also to arrange it onto chromosomal …

Technical advances have enabled the collection of genome and transcriptome data sets
with single-cell resolution. However, single-cell characterization of the epigenome has …

Genomes assembled de novo from short reads are highly fragmented relative to the finished
chromosomes of Homo sapiens and key model organisms generated by the Human …

Although we can increasingly measure transcription, chromatin, methylation, and other
aspects of molecular biology at single-cell resolution, most assays survey only one aspect of …

We characterize and extend a highly efficient method for constructing shotgun fragment
libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation …

Linking regulatory DNA elements to their target genes, which may be located hundreds of
kilobases away, remains challenging. Here, we introduce Cicero, an algorithm that identifies co…

Single-cell RNA sequencing (scRNA-seq) distinguishes cell types, states and lineages within
the context of heterogeneous tissues. However, current single-cell data cannot directly link …

Nanopore sequencing of DNA is a single-molecule technique that may achieve long reads,
low cost and high speed with minimal sample preparation and instrumentation. Here, we …

The HeLa cell line was established in 1951 from cervical cancer cells taken from a patient,
Henrietta Lacks. This was the first successful attempt to immortalize human-derived cells in …