Background Slow and cumbersome laboratory diagnostics for Mycobacterium tuberculosis
complex (MTBC) risk delayed treatment and poor patient outcomes. Whole-genome …

Objectives Whole-genome sequencing potentially represents a single, rapid and cost-effective
approach to defining resistance mechanisms and predicting phenotype, and strain type, …

… The PCR conditions used were as follows: initial denaturation at 95C for 3 min; 18 cycles
of 95C for 15 s, 62C for 15 s, and 65C for 2.5 min; and a final extension at 65C for 5 min. …

… Carlos del Ojo Elias , Carlos del Ojo Elias … Meera Chand, Theresa Lamagni, Katharina
Kranzer, Jessica Hedge, Ginny Moore, Simon Parks, Samuel Collins, Carlos del Ojo Elias, Nada …

We developed a low-cost and reliable method of DNA extraction from as little as 1 ml of
early positive mycobacterial growth indicator tube (MGIT) cultures that is suitable for whole-…

Use of whole-genome sequencing (WGS) for routine mycobacterial species identification and
drug susceptibility testing (DST) is becoming a reality. We compared the performances of …

… Carlos del Ojo Elias , Carlos del Ojo Elias … Nicole Stoesser, Sivilay Xayaheuang, Manivanh
Vongsouvath, Koukeo Phommasone, Ivo Elliott, Carlos del Ojo Elias, Derrick W. Crook, Paul …

We show how positional markers can be used to encode genetic variation within a Burrows-Wheeler
Transform (BWT), and use this to construct a generalisation of the traditional “…

Whole-genome sequencing was used to determine whether the reductions in recurrence of
Clostridium difficile infection observed with fidaxomicin in pivotal phase 3 trials occurred by …

Background Diagnosing drug-resistance remains an obstacle to the elimination of tuberculosis.
Phenotypic drug-susceptibility testing is slow and expensive, and commercial genotypic …