User profiles for D. Dobnik

David Dobnik

National Institute of Biology
Verified email at nib.si
Cited by 1625

[HTML][HTML] Cold plasma, a new hope in the field of virus inactivation

…, I Gutierrez-Aguirre, G Primc, M Mozetič, D Dobnik - Trends in …, 2020 - cell.com
Viruses can infect all cell-based organisms, from bacteria to humans, animals, and plants.
They are responsible for numerous cases of hospitalization, many deaths, and widespread …

[HTML][HTML] Critical assessment of digital PCR for the detection and quantification of genetically modified organisms

T Demeke, D Dobnik - Analytical and bioanalytical chemistry, 2018 - Springer
… 1, the target concentration also depends on the partition volume (V d ). A dilution factor
for the original sample before PCR (D) is also considered in the equation. Essentially, three …

Digital PCR applications in the SARS-CoV-2/COVID-19 era: a roadmap for future outbreaks

R Nyaruaba, C Mwaliko, D Dobnik… - Clinical Microbiology …, 2022 - Am Soc Microbiol
… (D) Common mutation spots associated with SARS-CoV-2 variants of concern. … (D)
PMA-coupled RT-dPCR for the determination of viable cells (98, 99). The PMA dye enters inactivated/…

[HTML][HTML] Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection

D Dobnik, D Štebih, A Blejec, D Morisset, J Žel - Scientific reports, 2016 - nature.com
The advantages of the digital PCR technology are already well documented until now. One
way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy. …

Multiplex quantification of 12 European Union authorized genetically modified maize lines with droplet digital polymerase chain reaction

D Dobnik, B Spilsberg, A Bogožalec Košir… - Analytical …, 2015 - ACS Publications
Presence of genetically modified organisms (GMO) in food and feed products is regulated in
many countries. The European Union (EU) has implemented a threshold for labeling of …

[HTML][HTML] Accurate quantification and characterization of adeno-associated viral vectors

D Dobnik, P Kogovšek, T Jakomin, N Košir… - Frontiers in …, 2019 - frontiersin.org
One of the main challenges in the gene therapy viral vector development is to establish an
optimized process for its large scale production. This requires optimization for upstream and …

[HTML][HTML] Droplet volume variability as a critical factor for accuracy of absolute quantification using droplet digital PCR

…, C Divieto, J Pavšič, S Pavarelli, D Dobnik… - Analytical and …, 2017 - Springer
… The copy number concentration depends also on the partition volume (Vd) and the dilution
factor of the original solution in the PCR reaction (D). Consequently, the accuracy of absolute …

Antioxidant and antigenotoxic effects of rosemary (Rosmarinus officinalis L.) extracts in Salmonella typhimurium TA98 and HepG2 cells

B Žegura, D Dobnik, MH Niderl, M Filipič - Environmental toxicology and …, 2011 - Elsevier
In the present study the chemopreventive effects of water soluble AquaROX ® 15 and oil
soluble VivOX ® 40 rosemary extracts against 4-nitroquinoline-N-oxide (NQNO) and 2-amino-3-…

GMO quantification: valuable experience and insights for the future

M Milavec, D Dobnik, L Yang, D Zhang… - Analytical and …, 2014 - Springer
Cultivation and marketing of genetically modified organisms (GMOs) have been unevenly
adopted worldwide. To facilitate international trade and to provide information to consumers, …

NAIMA: target amplification strategy allowing quantitative on-chip detection of GMOs

D Morisset, D Dobnik, S Hamels, J Žel… - Nucleic acids …, 2008 - academic.oup.com
We have developed a novel multiplex quantitative DNA-based target amplification method
suitable for sensitive, specific and quantitative detection on microarray. This new method …