Bordetella pertussis secretes a calmodulin-activated adenylate cyclase toxin (CyaA) that is
able to deliver its amino-terminal catalytic domain into the cytosol of eukaryotic cells. The …

We describe a bacterial two-hybrid system that allows an easy in vivo screening and
selection of functional interactions between two proteins. This genetic test is based on the …

Formation of the Escherichia coli division septum is catalyzed by a number of essential proteins
(named Fts) that assemble into a ring-like structure at the future division site. Several of …

This book describes the major achievements and discoveries relevant to bacterial protein
toxins since the turn of the new century illustrated by the discovery of more than fifty novel …

… The cyaA gene is part of an operon that also contains the genes cyaB, -D, and -E, required
… The affinity of CyaA for D1 and J774A.1 cells was high since the apparent K d were 9.2 ± 4.5 …

The adenylate cyclase toxin of the prokaryote Bordetella pertussis is stimulated by the
eukaryotic regulatory protein, calmodulin. A general strategy, using the adenylate‐cyclase‐…

… (Detailed schemes of plasmid constructions are available upon request.) Unless otherwise
stated, pACT7 (Sebo et al., 1991) was used for construction of deletions in the cyaA gene. …

… Supported by the Agence Nationale de Recherches sur le SIDA.The abbreviations used
are: … Circular dichroism intensity (ΔA) was calibrated with an aqueous solution of d-(+)-10-…

… Growth of Lac ÷ colonies will be detected after 4-5 days of incubation at 30 . The X-Gal
substrate can also be added to the medium to facilitate the early visualization of Lac + comple…

We have recently developed a bacterial two-hybrid system (BACTH), based on functional
complementation between two fragments of the catalytic domain of Bordetella pertussis …