To broaden the range of tools available for proteomic research, we generated a library of
16,368 unique full-length human ORFs that are expressible as N-terminal GST-His 6 fusion …

Developmental switching between life‐cycle stages is a common feature among many
pathogenic organisms. The protozoan parasite Entamoeba histolytica converts between cysts (…

Yeast Tyl elements are retrotransposons that transpose via an RNA intermediate found in a
virus-like particle (Ty-VLP). A Ty-encoded reverse transcriptase activity found inside the …

A key component of efforts to address the reproducibility crisis in biomedical research is the
development of rigorously validated and renewable protein-affinity reagents. As part of the …

The gene encoding the circumsporozoite (CS) protein of the rodent malaria parasite
Plasmodium berghei was cloned and characterized. A cDNA library made from P. berghei …

Background Histone modification regulates chromatin structure and influences gene
expression associated with diverse biological functions including cellular differentiation, cancer, …

Monoclonal antibodies (mAbs) against human proteins are the primary protein capture
reagents for basic research, diagnosis, and molecular therapeutics. The 2 most important …

Entamoeba invadens is pathogenic in multiple reptile species and has caused severe
outbreaks in zoos and other facilities worldwide. Infections can be difficult to diagnose and to …

Background The immunoprecipitation (IP) assay is a valuable molecular biology tool applied
across a breadth of fields. The standard assay couples IP to immunoblotting (IP/IB), a …

Gastrointestinal obstructive complications after laparoscopic Roux-en-Y gastric bypass (LRYGBP)
are not uncommon. Their usual causes are strictures, internal hernias and adhesions. …