Recombineering and MAGE
…, PN Ciaccia, AD Ellington, GT Filsinger… - Nature Reviews …, 2021 - nature.com
Recombination-mediated genetic engineering, also known as recombineering, is the
genomic incorporation of homologous single-stranded or double-stranded DNA into bacterial …
genomic incorporation of homologous single-stranded or double-stranded DNA into bacterial …
Improved bacterial recombineering by parallelized protein discovery
…, M Czikkely, D Balogh, GT Filsinger… - Proceedings of the …, 2020 - National Acad Sciences
Exploiting bacteriophage-derived homologous recombination processes has enabled
precise, multiplex editing of microbial genomes and the construction of billions of customized …
precise, multiplex editing of microbial genomes and the construction of billions of customized …
Characterizing the portability of phage-encoded homologous recombination proteins
Efficient genome editing methods are essential for biotechnology and fundamental research.
Homologous recombination (HR) is the most versatile method of genome editing, but …
Homologous recombination (HR) is the most versatile method of genome editing, but …
Halide ion-mediated growth of single crystalline Fe nanoparticles
We report a facile halide ion (Cl− or Br−) mediated synthesis of Fe nanoparticles (NPs) by
thermal decomposition of Fe(CO)5. The NP structure is controlled to be either amorphous (in …
thermal decomposition of Fe(CO)5. The NP structure is controlled to be either amorphous (in …
Crystal structure of the Redβ C-terminal domain in complex with λ Exonuclease reveals an unexpected homology with λ Orf and an interaction with Escherichia coli …
BJ Caldwell, E Zakharova, GT Filsinger… - Nucleic acids …, 2019 - academic.oup.com
Bacteriophage λ encodes a DNA recombination system that includes a 5′-3′ exonuclease
(λ Exo) and a single strand annealing protein (Redβ). The two proteins form a complex that …
(λ Exo) and a single strand annealing protein (Redβ). The two proteins form a complex that …
[HTML][HTML] Optimizing complex phenotypes through model-guided multiplex genome engineering
We present a method for identifying genomic modifications that optimize a complex phenotype
through multiplex genome engineering and predictive modeling. We apply our method to …
through multiplex genome engineering and predictive modeling. We apply our method to …
Laboratory-generated DNA can cause anomalous pathogen diagnostic test results
…, AJ Mijalis, GT Filsinger… - Microbiology …, 2021 - Am Soc Microbiol
The coronavirus disease 2019 (COVID-19) pandemic has brought about the unprecedented
expansion of highly sensitive molecular diagnostics as a primary infection control strategy. …
expansion of highly sensitive molecular diagnostics as a primary infection control strategy. …
Anomalous COVID-19 tests hinder researchers
LR Robinson-McCarthy, AJ Mijalis, GT Filsinger… - Science, 2021 - science.org
Universities conduct a large proportion of the community surveillance testing for severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2)(1). At the same time, they have shifted …
respiratory syndrome coronavirus 2 (SARS-CoV-2)(1). At the same time, they have shifted …
Characterizing the portability of RecT-mediated oligonucleotide recombination
Bacterial genome editing methods are used to engineer strains for biotechnology and
fundamental research. Homologous recombination (HR) is the most versatile method of genome …
fundamental research. Homologous recombination (HR) is the most versatile method of genome …
New recommended policies for pathogen surveillance testing of researchers and improved stewardship of diagnostic DNA
L Robinson-McCarthy, AJ Mijalis, GT Filsinger… - 2020 - osf.io
The SARS-CoV-2 pandemic has brought about the unprecedented expansion of highly
sensitive molecular diagnostics as a primary infection control strategy. At the same time, many …
sensitive molecular diagnostics as a primary infection control strategy. At the same time, many …