Giant unilamellar vesicle formation under physiologically relevant conditions
T Pott, H Bouvrais, P Méléard - Chemistry and physics of lipids, 2008 - Elsevier
We present an upgrade to the giant unilamellar vesicle (GUV) electroformation method allowing
easy GUV production in different buffers and with various membrane compositions. Our …
easy GUV production in different buffers and with various membrane compositions. Our …
[HTML][HTML] Impact of membrane-anchored fluorescent probes on the mechanical properties of lipid bilayers
H Bouvrais, T Pott, LA Bagatolli, JH Ipsen… - Biochimica et Biophysica …, 2010 - Elsevier
Fluorescent probes are used in membrane biophysics studies to provide information about
physical properties such as lipid packing, polarity and lipid diffusion or to visualize membrane …
physical properties such as lipid packing, polarity and lipid diffusion or to visualize membrane …
Softening of POPC membranes by magainin
Magainin 2 belongs to the family of peptides, which interacts with the lipid membranes. The
present work deals with the effect of this peptide on the mechanical properties of 1-palmitoyl-…
present work deals with the effect of this peptide on the mechanical properties of 1-palmitoyl-…
Buffers affect the bending rigidity of model lipid membranes
H Bouvrais, L Duelund, JH Ipsen - Langmuir, 2014 - ACS Publications
In biophysical and biochemical studies of lipid bilayers the influence of the used buffer is
often ignored or assumed to be negligible on membrane structure, elasticity, or physical …
often ignored or assumed to be negligible on membrane structure, elasticity, or physical …
Advantages of statistical analysis of giant vesicle flickering for bending elasticity measurements
P Méléard, T Pott, H Bouvrais, JH Ipsen - The European Physical Journal …, 2011 - Springer
We show how to greatly improve precision when determining bending elasticity of giant
unilamellar vesicles. Taking advantage of the well-known quasi-spherical model of liposome …
unilamellar vesicles. Taking advantage of the well-known quasi-spherical model of liposome …
Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid–protein interactions of nonequilibrium membranes
H Bouvrais, F Cornelius, JH Ipsen… - Proceedings of the …, 2012 - National Acad Sciences
Interaction between integral membrane proteins and the lipid-bilayer component of biological
membranes is expected to mutually influence the proteins and the membrane. We present …
membranes is expected to mutually influence the proteins and the membrane. We present …
Bending rigidities of lipid bilayers: their determination and main inputs in biophysical studies
H Bouvrais - Advances in planar lipid bilayers and liposomes, 2012 - Elsevier
Because of the complexity of the biological membranes, artificial membranes are often used
as membrane models for biophysical studies. This is especially true for the investigation of …
as membrane models for biophysical studies. This is especially true for the investigation of …
Lipophilic contaminants influence cold tolerance of invertebrates through changes in cell membrane fluidity
M Holmstrup, H Bouvrais, P Westh… - … science & technology, 2014 - ACS Publications
Contaminants taken up by living organisms in the environment as a result of anthropogenic
contamination can reduce the tolerance of natural stressors, eg, low temperatures, but the …
contamination can reduce the tolerance of natural stressors, eg, low temperatures, but the …
[PDF][PDF] Microtubule feedback and LET-99-dependent control of pulling forces ensure robust spindle position
H Bouvrais, L Chesneau, S Pastezeur, D Fairbrass… - Biophysical …, 2018 - cell.com
… of 48 h, except for ima3(RNAi), which was treated for 34 h. … : the treatment lasted 6 h for spd-2
and gpr-1/2 and 24 h for par-3 … during 48 h, spd-2(RNAi) for 6 h, and let-99(RNAi) for 24 h. …
and gpr-1/2 and 24 h for par-3 … during 48 h, spd-2(RNAi) for 6 h, and let-99(RNAi) for 24 h. …
[HTML][HTML] Lack of chromokinesin Klp-19 creates a more rigid midzone and affects force transmission during anaphase in C. elegans
Recent studies have highlighted the significance of the spindle midzone–the region positioned
between chromosomes–in ensuring proper chromosome segregation. By combining …
between chromosomes–in ensuring proper chromosome segregation. By combining …