Giant unilamellar vesicle formation under physiologically relevant conditions

T Pott, H Bouvrais, P Méléard - Chemistry and physics of lipids, 2008 - Elsevier
We present an upgrade to the giant unilamellar vesicle (GUV) electroformation method allowing
easy GUV production in different buffers and with various membrane compositions. Our …

[HTML][HTML] Impact of membrane-anchored fluorescent probes on the mechanical properties of lipid bilayers

H Bouvrais, T Pott, LA Bagatolli, JH Ipsen… - Biochimica et Biophysica …, 2010 - Elsevier
Fluorescent probes are used in membrane biophysics studies to provide information about
physical properties such as lipid packing, polarity and lipid diffusion or to visualize membrane …

Softening of POPC membranes by magainin

H Bouvrais, P Méléard, T Pott, KJ Jensen, J Brask… - Biophysical …, 2008 - Elsevier
Magainin 2 belongs to the family of peptides, which interacts with the lipid membranes. The
present work deals with the effect of this peptide on the mechanical properties of 1-palmitoyl-…

Buffers affect the bending rigidity of model lipid membranes

H Bouvrais, L Duelund, JH Ipsen - Langmuir, 2014 - ACS Publications
In biophysical and biochemical studies of lipid bilayers the influence of the used buffer is
often ignored or assumed to be negligible on membrane structure, elasticity, or physical …

Advantages of statistical analysis of giant vesicle flickering for bending elasticity measurements

P Méléard, T Pott, H Bouvrais, JH Ipsen - The European Physical Journal …, 2011 - Springer
We show how to greatly improve precision when determining bending elasticity of giant
unilamellar vesicles. Taking advantage of the well-known quasi-spherical model of liposome …

Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid–protein interactions of nonequilibrium membranes

H Bouvrais, F Cornelius, JH Ipsen… - Proceedings of the …, 2012 - National Acad Sciences
Interaction between integral membrane proteins and the lipid-bilayer component of biological
membranes is expected to mutually influence the proteins and the membrane. We present …

Bending rigidities of lipid bilayers: their determination and main inputs in biophysical studies

H Bouvrais - Advances in planar lipid bilayers and liposomes, 2012 - Elsevier
Because of the complexity of the biological membranes, artificial membranes are often used
as membrane models for biophysical studies. This is especially true for the investigation of …

Lipophilic contaminants influence cold tolerance of invertebrates through changes in cell membrane fluidity

M Holmstrup, H Bouvrais, P Westh… - … science & technology, 2014 - ACS Publications
Contaminants taken up by living organisms in the environment as a result of anthropogenic
contamination can reduce the tolerance of natural stressors, eg, low temperatures, but the …

[PDF][PDF] Microtubule feedback and LET-99-dependent control of pulling forces ensure robust spindle position

H Bouvrais, L Chesneau, S Pastezeur, D Fairbrass… - Biophysical …, 2018 - cell.com
… of 48 h, except for ima3(RNAi), which was treated for 34 h. … : the treatment lasted 6 h for spd-2
and gpr-1/2 and 24 h for par-3 … during 48 h, spd-2(RNAi) for 6 h, and let-99(RNAi) for 24 h. …

[HTML][HTML] Lack of chromokinesin Klp-19 creates a more rigid midzone and affects force transmission during anaphase in C. elegans

…, B Mustafa, X Horton, K Siller, L Cueff, H Bouvrais… - bioRxiv, 2023 - ncbi.nlm.nih.gov
Recent studies have highlighted the significance of the spindle midzone–the region positioned
between chromosomes–in ensuring proper chromosome segregation. By combining …