For most proteins in the genome databases, function is predicted via sequence comparison.
In spite of the popularity of this approach, the extent to which it can be reliably applied is …

… at 80C, and added to GreeneChip for hybridization for 16 h at 65C. After 10-min washes at
room … were added and incubated at 65C for 1 h. Slides were washed as before, air dried, and …

Alternative premessenger RNA splicing enables genes to generate more than one gene
product. Splicing events that occur within protein coding regions have the potential to alter the …

Background Sequencing the genomes of the first few eukaryotes created the impression
that gene number shows no correlation with organism complexity, often referred to as the G-…

A thorough sequence analysis of the various members of the eukaryotic protein serine/threonine
phosphatase 2C (PP2C) family revealed the conservation of 11 motifs. These motifs …

We have developed an initial approach for annotating and surveying pseudogenes in the
human genome. We search human genomic DNA for regions that are similar to known protein …

… coli and H. influenzae revealed 116 genes that are present only in the latter [37]. Differential
… coli are missing in H. influenzae, and the metabolism in the latter seems to be biased to a …

Annotation transfer is a principal process in genome annotation. It involves “transferring”
structural and functional annotation to uncharacterized open reading frames (ORFs) in a newly …

Chromosomal translocations, which often generate chimeric proteins by fusing segments of
two distinct genes, represent the single major genetic aberration leading to cancer. We …

Background The idea that the assembly of protein complexes is linked with protein disorder
has been inferred from a few large complexes, such as the viral capsid or bacterial flagellar …