A general method to improve fluorophores for live-cell and single-molecule microscopy

JB Grimm, BP English, J Chen, JP Slaughter, Z Zhang… - Nature …, 2015 - nature.com
Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence
microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes …

A general method to fine-tune fluorophores for live-cell and in vivo imaging

JB Grimm, AK Muthusamy, Y Liang, TA Brown… - Nature …, 2017 - nature.com
Pushing the frontier of fluorescence microscopy requires the design of enhanced
fluorophores with finely tuned properties. We recently discovered that incorporation of four-membered …

Bright photoactivatable fluorophores for single-molecule imaging

JB Grimm, BP English, H Choi, AK Muthusamy… - Nature …, 2016 - nature.com
Small-molecule fluorophores are important tools for advanced imaging experiments. We
previously reported a general method to improve small, cell-permeable fluorophores which …

Synthesis of rhodamines from fluoresceins using Pd-catalyzed C–N cross-coupling

JB Grimm, LD Lavis - Organic letters, 2011 - ACS Publications
A unified, convenient, and efficient strategy for the preparation of rhodamines and N,N′-diacylated
rhodamines has been developed. Fluorescein ditriflates were found to undergo …

Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels

JB Grimm, LD Lavis - Nature Methods, 2022 - nature.com
The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed the …

The chemistry of small-molecule fluorogenic probes

JB Grimm, LM Heckman, LD Lavis - Progress in molecular biology and …, 2013 - Elsevier
Chemical fluorophores find wide use in biology to detect and visualize different phenomena.
A key advantage of small-molecule dyes is the ability to construct compounds where …

Bright and photostable chemigenetic indicators for extended in vivo voltage imaging

…, L Campagnola, SC Seeman, J Yu, J Zheng, JB Grimm… - Science, 2019 - science.org
Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high
spatial and temporal resolution. However, the utility of existing GEVIs has been limited by …

Real-time quantification of single RNA translation dynamics in living cells

…, JG DeLuca, BP English, Z Zhang, LD Lavis, JB Grimm… - Science, 2016 - science.org
Although messenger RNA (mRNA) translation is a fundamental biological process, it has
never been imaged in real time in vivo with single-molecule precision. To achieve this, we …

High-density three-dimensional localization microscopy across large volumes

WR Legant, L Shao, JB Grimm, TA Brown, DE Milkie… - Nature …, 2016 - nature.com
Extending three-dimensional (3D) single-molecule localization microscopy away from the
coverslip and into thicker specimens will greatly broaden its biological utility. However, …

[PDF][PDF] Steroid receptors reprogram FoxA1 occupancy through dynamic chromatin transitions

…, TS Karpova, D Ball, D Mazza, LD Lavis, JB Grimm… - Cell, 2016 - cell.com
The estrogen receptor (ER), glucocorticoid receptor (GR), and forkhead box protein 1 (FoxA1)
are significant factors in breast cancer progression. FoxA1 has been implicated in …