Recombineering: genetic engineering in bacteria using homologous recombination

LC Thomason, JA Sawitzke, X Li… - Current protocols in …, 2014 - Wiley Online Library
The bacterial chromosome and bacterial plasmids can be engineered in vivo by homologous
recombination using PCR products and synthetic oligonucleotides as substrates. This is …

Genetic engineering using homologous recombination

DL Court, JA Sawitzke… - Annual review of genetics, 2002 - annualreviews.org
▪ Abstract In the past few years, in vivo technologies have emerged that, due to their efficiency
and simplicity, may one day replace standard genetic engineering techniques. Constructs …

Recombineering: in vivo genetic engineering in E. coli, S. enterica, and beyond

JA Sawitzke, LC Thomason, N Costantino… - Methods in …, 2007 - Elsevier
… the recombinant produces altered colony morphology or a slow‐growth phenotype, these
can be detected directly by looking for that minority class of colonies (Thomason and Sawitzke, …

Evidence of association of APOE with age‐related macular degeneration ‐ a pooled analysis of 15 studies

…, AD Hingorani, R Sofat, M Dean, J Sawitzke… - Human …, 2011 - Wiley Online Library
Age‐related macular degeneration (AMD) is the most common cause of incurable visual
impairment in high‐income countries. Previous studies report inconsistent associations …

Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli

X Li, LC Thomason, JA Sawitzke… - Nucleic acids …, 2013 - academic.oup.com
The two-step process of selection and counter-selection is a standard way to enable genetic
modification and engineering of bacterial genomes using homologous recombination …

Probing cellular processes with oligo-mediated recombination and using the knowledge gained to optimize recombineering

JA Sawitzke, N Costantino, X Li, LC Thomason… - Journal of molecular …, 2011 - Elsevier
Recombination with single-strand DNA oligonucleotides (oligos) in Escherichia coli is an
efficient and rapid way to modify replicons in vivo. The generation of nucleotide alteration by …

Suppression of chromosome segregation defects of Escherichia coli muk mutants by mutations in topoisomerase I

JA Sawitzke, S Austin - Proceedings of the National …, 2000 - National Acad Sciences
Escherichia coli muk mutants are temperature-sensitive and produce anucleate cells. A
spontaneously occurring mutation was found in a ΔmukB∷kan mutant strain that suppressed the …

Variations in apolipoprotein E frequency with age in a pooled analysis of a large group of older people

…, PTDV De Jong, M Dean, J Sawitzke… - American journal of …, 2011 - academic.oup.com
Variation in the apolipoprotein E gene (APOE) has been reported to be associated with
longevity in humans. The authors assessed the allelic distribution of APOE isoforms ε2, ε3, and …

Genome analysis of Latin American cervical cancer: frequent activation of the PIK3CA pathway

…, J Mitchell, D Roberson, J Sawitzke… - Clinical Cancer …, 2015 - AACR
Purpose: Cervical cancer is one of the most common causes of cancer mortality for women
living in poverty, causing more than 28,000 deaths annually in Latin America and 266,000 …

Recombineering with overlapping single-stranded DNA oligonucleotides: testing a recombination intermediate

D Yu, JA Sawitzke, H Ellis… - Proceedings of the …, 2003 - National Acad Sciences
A phage λ-based recombination system, Red, can be used for high-efficiency mutagenesis,
repair, and engineering of chromosomal or episomal DNA in vivo in Escherichia coli. When …