… ij , normalized relative quantity from qbase PLUS for sample i and assay j; CN ij , diploid
copy number of the locus measured by assay j in sample i). When using only a single reference …

Gene expression analyses of bulk tissues often ignore cell type composition as an important
confounding factor, resulting in a loss of signal from lowly abundant cell types. In this review…

Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

… C q r e f e r n c e , j l = C q j l = ∑ k = 1 s C q j k l s ( formula 9 ) MathType@MTEF@5@5@+=…
Calculation of the calibration factor CF for gene j in run l based on the NRQs of the IRCs m: …

… For every combination of two internal control genes j and k, an array … j and k as the standard
deviation of the A jk elements (Equation 3). The gene-stability measure M j for control gene j …

Small-cell lung cancer (SCLC) is an aggressive lung tumor subtype with poor prognosis 1 ,
2 , 3 . We sequenced 29 SCLC exomes, 2 genomes and 15 transcriptomes and found an …

In recent years, technological advances in transcriptome profiling revealed that the repertoire
of human RNA molecules is more diverse and extended than originally thought. This …

MicroRNAs (miRNAs) are increasingly implicated in regulating the malignant progression of
cancer. Here we show that miR-9, which is upregulated in breast cancer cells, directly …

We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting
to facilitate interpretation and replication of experiments. To achieve this, we describe EV-…

There is growing interest in digital PCR (dPCR) because technological progress makes it a
practical and increasingly affordable technology. dPCR allows the precise quantification of …