The microbial adaptive immune system CRISPR mediates defense against foreign genetic
elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi…

Systematic interrogation of gene function requires the ability to perturb gene expression in a
robust and generalizable manner. Here we describe structure-guided engineering of a …

Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen
detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered …

INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …

Rapid detection of nucleic acids is integral for clinical diagnostics and biotechnological
applications. We recently developed a platform termed SHERLOCK (specific high-sensitivity …

RNA has important and diverse roles in biology, but molecular tools to manipulate and
measure it are limited. For example, RNA interference 1 , 2 , 3 can efficiently knockdown RNAs, …

Nucleic acid editing holds promise for treating genetic disease, particularly at the RNA level,
where disease-relevant sequences can be rescued to yield functional protein products. …

Microbial CRISPR-Cas systems are divided into Class 1, with multisubunit effector complexes,
and Class 2, with single protein effectors. Currently, only two Class 2 effectors, Cas9 and …

To achieve its precise neural connectivity, the developing mammalian nervous system
undergoes extensive activity-dependent synapse remodelling. Recently, microglial cells have …

Detection of SARS-CoV-2 with a CRISPR-Based Test A new method for the detection of
SARS-CoV-2 combines simplified extraction of RNA with isothermal amplification and CRISPR (…