User profiles for K. McJunkin

Katherine McJunkin

NIDDK Intramural Research Program
Verified email at nih.gov
Cited by 3081

miR-221 overexpression contributes to liver tumorigenesis

P Pineau, S Volinia, K McJunkin… - Proceedings of the …, 2010 - National Acad Sciences
K.-1 cells were transfected with vector expressing miR-221, miR-222, or empty vector,
and protein levels were checked by Western blotting with the indicated antibodies. One …

Genome-wide RNA-mediated interference screen identifies miR-19 targets in Notch-induced T-cell acute lymphoblastic leukaemia

…, T Palomero, K De Keersmaecker, K McJunkin… - Nature cell …, 2010 - nature.com
K over-represented shRNAs from the screen, in comparison with the full shRNA library. We
used values of K … by a Fisher's exact test for any value of K (P >0.05 in all cases for each gene)…

In vivo RNAi screening identifies a mechanism of sorafenib resistance in liver cancer

R Rudalska, D Dauch, T Longerich, K McJunkin… - Nature medicine, 2014 - nature.com
In solid tumors, resistance to therapy inevitably develops upon treatment with cytotoxic drugs
or molecularly targeted therapies. Here, we describe a system that enables pooled shRNA …

Toolkit for evaluating genes required for proliferation and survival using tetracycline-regulated RNAi

J Zuber, K McJunkin, C Fellmann, LE Dow… - Nature …, 2011 - nature.com
Short hairpin RNAs (shRNAs) are versatile tools for analyzing loss-of-function phenotypes
in vitro and in vivo 1 . However, their use for studying genes involved in proliferation and …

[PDF][PDF] Functional identification of optimized RNAi triggers using a massively parallel sensor assay

C Fellmann, J Zuber, K McJunkin, K Chang… - Molecular cell, 2011 - cell.com
… to nonscoring shRNAs and flanking mRNA regions, the nucleotide composition of potent
guide strands shows many significant positional biases (p < 0.01, Pearson’sc2 test with Šidá k

Recent advances in understanding microRNA function and regulation in C. elegans

K Kotagama, K McJunkin - Seminars in Cell & Developmental Biology, 2023 - Elsevier
MicroRNAs (miRNAs) were first discovered in C. elegans as essential post-transcriptional
regulators of gene expression. Since their initial discovery, miRNAs have been implicated in …

A pipeline for the generation of shRNA transgenic mice

…, PK Premsrirut, J Zuber, C Fellmann, K McJunkin… - Nature protocols, 2012 - nature.com
RNA interference (RNAi) is an extremely effective tool for studying gene function in almost
all metazoan and eukaryotic model systems. RNAi in mice, through the expression of short …

Tissue-specific and reversible RNA interference in transgenic mice

RA Dickins, K McJunkin, E Hernando, PK Premsrirut… - Nature …, 2007 - nature.com
Genetically engineered mice provide powerful tools for understanding mammalian gene
function. These models traditionally rely on gene overexpression from transgenes or targeted, …

CRISPR screening strategies for microRNA target identification

B Yang, K McJunkin - The FEBS Journal, 2020 - Wiley Online Library
Identifying microRNA (miRNA) target genes remains a major challenge in understanding
the roles miRNAs play in gene regulation. Furthermore, understanding which miRNA–target …

A microRNA family exerts maternal control on sex determination in C. elegans

K McJunkin, V Ambros - Genes & development, 2017 - genesdev.cshlp.org
Gene expression in early animal embryogenesis is in large part controlled post-transcriptionally.
Maternally contributed microRNAs may therefore play important roles in early …