Fluoropyrimidines are the first-line treatment for colorectal cancer, but their efficacy is highly
variable between patients. We queried whether gut microbes, a known source of inter-…

Microbial communities are composed of cells of varying metabolic capacity, and regularly
include auxotrophs that lack essential metabolic pathways. Through analysis of auxotrophs for …

… −1 CSM-His-Leu-Met-Trp-Ura (powder; MP Biomedicals), 60 mg l −1 leucine, 20 mg l −1
methionine, 40 mg l −1 tryptophan and 20 mg l −1 uracil) or SC-Ura (2% (wt/vol) glucose, 6.8 g l …

Both single and multicellular organisms depend on anti-stress mechanisms that enable them
to deal with sudden changes in the environment, including exposure to heat and oxidants. …

Metabolism is deeply intertwined with aging. Effects of metabolic interventions on aging
have been explained with intracellular metabolism, growth control, and signaling. Studying …

Advances in our ability to systematically introduce and track controlled genetic variance in
microorganisms have, in the past decade, fuelled high-throughput reverse genetics …

… Most of the conditions (N = 197) were tested at the same time and under the same
laboratory conditions as the KEIO KO collection (Herrera-Dominguez et al., unpublished). …

Functional genomic strategies have become fundamental for annotating gene function and
regulatory networks. Here, we combined functional genomics with proteomics by quantifying …

All biosynthetically active cells release metabolites, in part due to membrane leakage and
cell lysis, but also in part due to overflow metabolism and ATP-dependent membrane export. …

… Importantly, we identify that nisin degradation by NSR is frequent among L. lactis and
therefore likely the main mechanism by which dairy-associated L. lactis strains tolerate nisin. …