CRISPR‐Cas systems have rapidly transitioned from intriguing prokaryotic defense systems
to powerful and versatile biomolecular tools. This article reviews how these systems have …

Currently, porcine circovirus type 2 (PCV2) is considered the major pathogen of porcine
circovirus associated-diseases (PCVAD) that causes large economic losses for the swine …

… The Newcastle-Ottawa Scale 15 (NOS) were used to assess the quality of the included
studies by two reviewers (QZ and ML). This scale ranged from 0 (poor) to 9 stars (excellent) and …

… A 1.25 ml sample of Ti(OCH(CH 3 ) 2 ) 4 (AR; Chengdu Unite-chemcial Company, China)
dissolved in 25 ml of absolute ethanol was dropped to 250 ml of distilled water (4 C), adjusted …

… Antibiotics were administered at the following final concentrations: 50 µg/ml streptomycin,
50 µg/ml kanamycin, 50 µg/ml ampicillin, 2 µg/ml chloramphenicol, and 2 µg/ml erythromycin. …

… mAbs were produced by injecting 2 ml of 2.5 × 10 6 cells per ml hybridoma cells into
nude mice intraperitoneally to collect ascites, followed by purifying mAbs from ascites using …

… Cultures were back-diluted 1:25 into 25 ml of LB medium in 125-ml Erlenmeyer flasks
and grown to an ABS 600 of 0.6–0.8, which was quantified using a Nanodrop 2000c …

… of this dilution was then used as the DNA template in a 50 ml PCR reaction with 55 C …
into 2 mL of LB with 34 mg/ml chloramphenicol (Cm), 50 mg/ml ampicillin (Amp), and 50 mg/ml …

… In the stable transfection, cells were selected with 200 μg/mL G418, and clones were isolated
by serial dilution. Three CTTN RNAi clones (C1, C6, and C19) and three scramble RNAi …

… 2–21 μIU/ml (14–144 pmol/liter), with a mean of 11 μIU/ml (79 … μIU/ml (54 pmol/liter) and
4.2% at a level of 17 μIU/ml (123 … μIU/ml (85 pmol/liter) and 8.5% for values near 45 μIU/ml (324 …