Oocytes, the female germ cells, contain all the messenger RNAs necessary to start a new
life but typically wait until fertilization to begin development. The transition from oocyte to …

The genetic architecture of common traits, including the number, frequency, and effect sizes
of inherited variants that contribute to individual risk, has been long debated. Genome-wide …

Chromatin-based functional genomic analyses and genomewide association studies (GWASs)
together implicate enhancers as critical elements influencing gene expression and risk …

… We thank Palucka laboratory members Jan Martinek and Tina Wu for help on in situ RNA
analyses and members of the Stitzel, Ucar, and Parker laboratories, Travis Hinson, Karolina …

Pancreatic islet dysfunction and beta cell failure are hallmarks of type 2 diabetes mellitus (T2DM)
pathogenesis. In this review, we discuss how genome-wide association studies (…

… Islets were washed with prewarmed 1X PBS once and resuspended with dissociation
solution (1 ml of 1X PBS, 50 ml of 0.05 U/ml Dispase I stock solution [Roche; Indianapolis, IN]). …

Genome-wide association studies (GWAS) have identified >100 independent SNPs that
modulate the risk of type 2 diabetes (T2D) and related traits. However, the pathogenic …

… gently washed two times in PBS and then lysed using 1.0 mL 1% Nonidet P-40 lysis buffer
[1… Lysed cells were scraped and transferred into a 1.5-mL microcentrifuge tube. Extracts were …

… A titer of 1.2 ELISA units/ml or greater in a sample was predetermined by the manufacturer
… culture medium supplemented or not with 100 ng/ml IL-7 (PeproTech). After stimulation, cells …

We used an unbiased genome-wide approach to identify exonic variants segregating with
diabetes in a multigenerational Finnish family. At least eight members of this family presented …