… that each protein adopts a regular helical structure to form a canonical anti-parallel coiled-coil
interface with an observed disassociation constant in the low nanomolar region (12.4 nM). …

… CD spectra were collected from 185 to 260 nm (0.5 nm interval, 24 nm/min, 0.1 cm path length…
222 nm at 1C intervals over a temperature range of 10–94C with a heating rate of 1C /min. …

Although highly homologous to other methylcytosine-binding domain (MBD) proteins, MBD3
does not selectively bind methylated DNA, and thus the functional role of MBD3 remains in …

Coupling molecular biology to high-throughput sequencing has revolutionized the study of
biology. Molecular genomics techniques are continually refined to provide higher resolution …

Genome-wide association studies (GWAS) have discovered thousands loci associated with
disease risk and quantitative traits, yet most of the variants responsible for risk remain …

… scanning from 190 to 260 nm with 0.5-nm interval at a scanning speed of 50 nm/min. CD
spectra … the ratio of the observed θ 222 nm to the expected θ 222 nm for 100% helix as given by …

Unlike other members of the methyl-cytosine binding domain (MBD) family, MBD4 serves as
a potent DNA glycosylase in DNA mismatch repair specifically targeting m CpG/TpG …

… at 460 nm and HaloTag® emission was read at 618 nm. Raw values were converted to
milliBRET units (mBU) by taking 1,000 times the ratio of 618 nm emission to 460 nm emission. …

… We stained differentiated adipocytes with Oil Red O and measured absorbance at 540
nm to quantify lipid uptake. Lipid accumulation is a cellular phenotype that acts as a proxy …

… , AH109 clones co-transformed with indicated pairs of PACRG/pGADT7 and wild-type or
mutant MEIG1/pGBKT7 were cultured in SCM-Leu-Trp medium to optical density (OD) 600 nm –…