User profiles for N. Lubock
Nathan B. LubockOctant Verified email at octant.bio Cited by 698 |
High-resolution measurement of electrically-evoked vagus nerve activity in the anesthetized dog
… The signal-to-noise ratios (SNR) of electrically evoked CNAPs were calculated in two groups
of dogs in which the epineurium remained intact (n = 5) or was surgically removed (n = 5). …
of dogs in which the epineurium remained intact (n = 5) or was surgically removed (n = 5). …
Structural and functional characterization of G protein–coupled receptors with deep mutational scanning
… Lastly, we included several sequence elements in the donor vector to improve signal-to-noise
of the assay: an insulator upstream of the CRE reporter and an N-terminal affinity tag to the …
of the assay: an insulator upstream of the CRE reporter and an N-terminal affinity tag to the …
Multiplexed gene synthesis in emulsions for exploring protein functional landscapes
Improving our ability to construct and functionally characterize DNA sequences would
broadly accelerate progress in biology. Here, we introduce DropSynth, a scalable, low-cost …
broadly accelerate progress in biology. Here, we introduce DropSynth, a scalable, low-cost …
[HTML][HTML] Massively scaled-up testing for SARS-CoV-2 RNA via next-generation sequencing of pooled and barcoded nasal and saliva samples
…, EM Jones, M Gasperini, NB Lubock… - Nature Biomedical …, 2021 - nature.com
… Microbiology Laboratory comprising n = 94 SARS-CoV-2-positive samples and n = 286 …
samples collected into either normal saline (n = 128) or TE (n = 170). We showed 96.0% overall …
samples collected into either normal saline (n = 128) or TE (n = 170). We showed 96.0% overall …
[HTML][HTML] Swab-Seq: A high-throughput platform for massively scaled up SARS-CoV-2 testing
…, C Munugala, EM Jones, M Gasperini, NB Lubock… - MedRxiv, 2020 - ncbi.nlm.nih.gov
… (n=128) or TE (n=170). We showed 96.0% overall agreement, 92.0% positive percent
agreement and 97.6% negative percent agreement for all samples and a high correlation (r 2 =0.68…
agreement and 97.6% negative percent agreement for all samples and a high correlation (r 2 =0.68…
DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions
Multiplexed assays allow functional testing of large synthetic libraries of genetic elements,
but are limited by the designability, length, fidelity and scale of the input DNA. Here, we …
but are limited by the designability, length, fidelity and scale of the input DNA. Here, we …
[PDF][PDF] A scalable, multiplexed assay for decoding GPCR-ligand interactions with RNA sequencing
G protein-coupled receptors (GPCRs) are central to how mammalian cells sense and respond
to chemicals. Mammalian olfactory receptors (ORs), the largest family of GPCRs, mediate …
to chemicals. Mammalian olfactory receptors (ORs), the largest family of GPCRs, mediate …
A systematic comparison of error correction enzymes by next-generation sequencing
… We also removed any read pairs that had an ‘N’ base call in either one of the reads during
this step. We then took the filtered reads and merged read pairs with perfectly overlapping …
this step. We then took the filtered reads and merged read pairs with perfectly overlapping …
Genome-wide Functional Characterization of Escherichia coli Promoters and Regulatory Elements Responsible for their Function
Despite decades of intense genetic, biochemical, and evolutionary characterizations of
bacterial promoters, we lack the ability to identify or predict transcriptional activities of promoters …
bacterial promoters, we lack the ability to identify or predict transcriptional activities of promoters …
[HTML][HTML] Activation of peripheral nerve fibers by electrical stimulation in the sole of the foot
Background Human nociceptive withdrawal reflexes (NWR) can be evoked by electrical
stimulation applied to the sole of the foot. However, elicitation of NWRs is highly site dependent, …
stimulation applied to the sole of the foot. However, elicitation of NWRs is highly site dependent, …