The two major cellular sites for membrane protein degradation are the proteasome and the
lysosome. Ubiquitin attachment is a sorting signal for both degradation routes. For lysosomal …

Recent data both from cell-free experiments and from cultured cells have shown that
lysosomes can fuse directly with late endosomes to form a hybrid organelle. This has a led to a …

When ubiquitin (Ub) is attached to membrane proteins on the plasma membrane, it directs
them through a series of sorting steps that culminate in their delivery to the lumen of the …

This report compares the use of the lactate dehydrogenase (pLDH) assay with 3H-hypoxanthine
incorporation and Giemsa microscopy for the evaluation of anti-malaria drug inhibition …

Membrane proteins that are degraded in the vacuole of Saccharomyces cerevisiae are
sorted into discrete intralumenal vesicles, analogous to the internal membranes of multi-…

Phosphatidylinositol 3,5‐bisphosphate (PtdIns(3,5)P 2 ), made by Fab1p, is essential for
vesicle recycling from vacuole/lysosomal compartments and for protein sorting into …

Newly synthesized vacuolar hydrolases such as carboxypeptidase Y (CPY) are sorted from
the secretory pathway in the late-Golgi compartment and reach the vacuole after a distinct …

In E1-E2-E3 ubiquitin (Ub) conjugation cascades, the E2 first forms a transient E2∼Ub covalent
complex and then interacts with an E3 for Ub transfer. For cascades involving E3s in the …

A previously unreported afebrile, infectious leukoencephalomyelitis of one- to four-month-old
dairy goats was studied. The disease was characterized by pleocytosis and posterior …

Both heterotypic and homotypic fusion events are required to deliver endocytosed
macromolecules to lysosomes and remodel late endocytic organelles. A trans‐SNARE complex …