… b Scheme of expression plasmids for intracellular protein production (Biedendieck et al.
2007b). c Scheme of expression plasmids for extracellular protein production (Biedendieck et al. …

… and Meinhardt 1995), are best suited candidates for plasmid-based genetic applications,
including the generation of mutants and the recombinant production of proteins (Biedendieck et …

Bacillus megaterium is deep-rooted in the Bacillus phylogeny, making it an evolutionarily
key species and of particular importance in understanding genome evolution, dynamics, and …

Native cell-free transcription–translation systems offer a rapid route to characterize the
regulatory elements (promoters, transcription factors) for gene expression from nonmodel …

The high industrial relevance of the soil bacterium Bacillus megaterium as host for recombinant
proteins is driving systems-wide analyses of its metabolic and regulatory networks. The …

The sustainable production of industrial platform chemicals is one of the great challenges
facing the biotechnology field. Ideally, fermentation feedstocks would rather rely on industrial …

A novel levansucrase was identified in the supernatant of a cell culture of Bacillus
megaterium DSM319. In order to test for the contribution of specific amino acid residues to …

… revealed an identical elution point as the marker proteins yeast alcohol dehydrogenase (M
r = 150,000) indicating a M r of ∼150,000 and a hexameric oligomerization state. A UV-Vis …

It has been known for the past 20 years that two pathways exist in nature for the de novo
biosynthesis of the coenzyme form of vitamin B 12 , adenosylcobalamin, representing aerobic …

… The voting/consensus model was written in R script and Java. The code is freely available
at https://sourceforge.net/projects/insilico-fragmentation/. The application of the voting/…